Anti-S100A9 抗体 (ab81522)
Key features and details
- Goat polyclonal to S100A9
- Suitable for: WB, ICC, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-S100A9 antibody
S100A9 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to S100A9 -
由来種
Goat -
アプリケーション
適用あり: WB, ICC, IHC-P, Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Cow -
免疫原
Synthetic peptide:
C-DTNADKQLSFEEF
, corresponding to internal sequence amino acids 67-79 of Human S100A9 according to NP_002956.1 -
ポジティブ・コントロール
- Flow Cyt (Intra): MCF7 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81522の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 13 kDa.
1 hour primary incubation is recommended for this product. |
|
ICC |
Use a concentration of 10 µg/ml.
|
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IHC-P |
Use a concentration of 2.5 µg/ml.
|
|
Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 13 kDa. 1 hour primary incubation is recommended for this product. |
ICC
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 2.5 µg/ml. |
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions. -
組織特異性
Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses. -
配列類似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
翻訳後修飾
Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization. -
細胞内局在
Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation. - Information by UniProt
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参照データベース
- Entrez Gene: 532569 Cow
- Entrez Gene: 6280 Human
- Omim: 123886 Human
- SwissProt: P28783 Cow
- SwissProt: P06702 Human
- Unigene: 112405 Human
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別名
- Leukocyte L1 complex heavy chain antibody
- 60B8AG antibody
- CAGB antibody
see all
画像
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Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr at 10ug/mL, followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocyohemistry analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mL, showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10ug/mL, followed by Alexa Fluor 488 secondary antibody at 2ug/mL.
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Immunocytochemistry analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mL, showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mlL.
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All lanes : Anti-S100A9 antibody (ab81522) at 1 µg/ml
Lane 1 : Human bone marrow
Lane 2 : Gastrointestinal cancer lysate
Lane 3 : negative control HepG2 lysate.
Predicted band size: 13 kDa -
Immunohistochemical analysis of paraffin embedded human lung tissue labelling S100A9 with ab81522 at 2.5μg/ml.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab81522 は 1 報の論文で使用されています。
- Tsai SY et al. DAMP molecule S100A9 acts as a molecular pattern to enhance inflammation during influenza A virus infection: role of DDX21-TRIF-TLR4-MyD88 pathway. PLoS Pathog 10:e1003848 (2014). ELISA . PubMed: 24391503