Anti-S100A10 抗体 [EPR3317] (ab76472)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3317] to S100A10
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-S100A10 antibody [EPR3317]
S100A10 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3317] to S100A10 -
由来種
Rabbit -
特異性
The mouse recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse samples.
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アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human S100A10 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P60903 -
ポジティブ・コントロール
- WB: HaCaT, A431, SW480, RAW264.7, NIH/3T3, and HT-29 cell lysates; IHC-P: Human squamous cell cervical carcinoma, colon tissue and human kidney tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3317 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76472の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000 - 1/50000. Predicted molecular weight: 11 kDa.
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IHC-P | (2) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/5000 - 1/50000. Predicted molecular weight: 11 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase. -
配列類似性
Belongs to the S-100 family. - Information by UniProt
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参照データベース
- Entrez Gene: 6281 Human
- Entrez Gene: 20194 Mouse
- Omim: 114085 Human
- SwissProt: P60903 Human
- SwissProt: P08207 Mouse
- Unigene: 143873 Human
- Unigene: 1 Mouse
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別名
- 42C antibody
- AA409961 antibody
- AL024248 antibody
see all
画像
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All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/10000 dilution (unpurified)
Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible non-specific binding)
Exposure time: 180 secondsBlocking buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human squamous cell cervical carcinoma with purified ab76472 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Lane 1 : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/1000 dilution (purified)
Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/5000 dilution
All lanes :Recombinant Human S100A10 protein (His tag) (ab221399)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa (possible dimer)
Exposure time: 3 secondsBlocking buffer: 5% NFDM/TBST
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All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/50000 dilution (purified)
Lane 1 : HACAT cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HT-29 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/5000 dilution (purified)
Lane 1 : RAW264.7 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Unpurified ab76472 at 1/100 dilution staining S100A10 in human colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab76472 は 6 報の論文で使用されています。
- Wan T et al. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice. Nat Commun 13:1134 (2022). PubMed: 35241660
- Wang S et al. CSE/H2S ameliorates colitis in mice via protection of enteric glial cells and inhibition of the RhoA/ROCK pathway. Front Immunol 13:966881 (2022). PubMed: 36189321
- Lu MD et al. LINC01436 Promotes the Progression of Gastric Cancer via Regulating miR-513a-5p/APE1 Axis. Onco Targets Ther 13:10607-10619 (2020). PubMed: 33116638
- Zhao JT et al. LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR-320/S100A10 axis. Cell Biochem Funct 38:859-869 (2020). PubMed: 32128852
- Cheung TT et al. Annexin II Light Chain p11 Interacts With ENaC to Increase Functional Activity at the Membrane. Front Physiol 10:7 (2019). PubMed: 30800070
- Gocheva V et al. Quantitative proteomics identify Tenascin-C as a promoter of lung cancer progression and contributor to a signature prognostic of patient survival. Proc Natl Acad Sci U S A 114:E5625-E5634 (2017). PubMed: 28652369