Anti-S100 抗体 (ab34686)
Key features and details
- Rabbit polyclonal to S100
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-S100 antibody
S100 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to S100 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Cow, Human
交差が予測される動物種: Dog, Non human primates, Mammals -
免疫原
Full length native protein (purified): MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKELLQT ELSGFLDAQK DADAVDKVMK ELDENGDGEV DFQEYVVLVA ALTVACNNFF WENS, corresponding to amino acids 1-94 of Cow S100 (alpha chain). A mixture of alpha alpha homodimers and alpha beta heterodimers was used.
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特記事項
Please note that lot 296545 is supplied at 0.5mg/ml.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
This antibody was purified from monospecific antiserum. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34686の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1.67 - 10 µg/ml. Detects a band of approximately 11 kDa.
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IHC-P | (4) |
Use a concentration of 2.5 - 25 µg/ml.
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特記事項 |
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WB
Use a concentration of 1.67 - 10 µg/ml. Detects a band of approximately 11 kDa. |
IHC-P
Use a concentration of 2.5 - 25 µg/ml. |
ターゲット情報
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機能
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
組織特異性
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
配列類似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 431787 Cow
- Entrez Gene: 6271 Human
- Entrez Gene: 20193 Mouse
- Entrez Gene: 295214 Rat
- Omim: 176940 Human
- SwissProt: P02639 Cow
- SwissProt: P23297 Human
- SwissProt: P56565 Mouse
see all -
別名
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
画像
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ab34686 staining S100 in Rat nerve tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a 10mM citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/250 in PBS + 1x casein) for 1 hour at 25°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
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All lanes : Anti-S100 antibody (ab34686) at 1/1000 dilution
Lane 1 : Bovine S100 monomer at 0.1 µg
Lane 2 : S100 from rat brain lysate at 35 µg
Secondary
All lanes : IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX
Observed band size: 11 kDa why is the actual band size different from the predicted? -
Ab34686, at a 1/500 dilution, staining S100 in mouse brain tissue by immunohistochemistry. The tissue was formalin fixed and embedded in paraffin. Dark nuclear staining is observed within basket cells located near the Purkinje cells in the cerebellum.
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Ab34686, at 1/500 dilution, staining S100 in formalin fixed and paraffin embedded human brain astrocyte tumor tissue by immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab34686 は 7 報の論文で使用されています。
- Shibata O et al. Establishment of a pancreatic cancer animal model using the pancreas-targeted hydrodynamic gene delivery method. Mol Ther Nucleic Acids 28:342-352 (2022). PubMed: 35474735
- Su Y et al. Small Intestinal Submucosa Biomimetic Periosteum Promotes Bone Regeneration. Membranes (Basel) 12:N/A (2022). PubMed: 35877922
- Kakuta W et al. Regeneration of sensory nerve branches in extraction socket and surrounding alveolar bone in rat: immunohistochemical observation of the axon and myelin sheath changes. Odontology N/A:N/A (2022). PubMed: 36460825
- Wai V et al. The Anatomical Distribution of Mechanoreceptors in Mouse Hind Paw Skin and the Influence of Integrin a1ß1 on Meissner-Like Corpuscle Density in the Footpads. Front Neuroanat 15:628711 (2021). PubMed: 33737870
- Sohn EJ et al. Involvement of the miR-363-5p/P2RX4 Axis in Regulating Schwann Cell Phenotype after Nerve Injury. Int J Mol Sci 22:N/A (2021). PubMed: 34769029
- Gao L et al. Targeted next-generation sequencing of dedifferentiated chondrosarcoma in the skull base reveals combined TP53 and PTEN mutations with increased proliferation index, an implication for pathogenesis. Oncotarget 7:43557-43569 (2016). PubMed: 27248819
- Hsu M & Stevenson FF Reduction in muscular motility by selective focused cold therapy: a preclinical study. J Neural Transm 121:15-20 (2014). IHC-P ; Rat . PubMed: 23917804