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Full length native protein (purified) corresponding to Human S100 alpha. Purified S100-alpha protein from human pectoral muscle cells.
Our Abpromise guarantee covers the use of ab11428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). Use at a concentration of 0.1 µg/ml. Detects a band of approximately 10 kDa, representing S100-alpha from human muscle. S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.|
|IHC-P||Use a concentration of 1 µg/ml.|
|ICC||1/50 - 1/200.|
|ICC/IF||1/50 - 1/200.|
ab11428 labelling S100 alpha (red) in HEK293T cells by Immunocytochemistry/Immunofluorescence. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were incubated with primary antibody (1:100 in blocking buffer) overnight at 4ºC. A fluorophore-conjugated goat anti-rabbit IgG (1:200) was used as the secondary antibody (1 hour at room temperature). Blue (DAPI) - nuclei. Images were taken at 40X magnification.
Submitted by John Andersen
A Western blot of S100-alpha from human ovary extract (50µg) using ab11428 at 1/1000 dilution. A secondary goat anti rabbit HRP conjugate was used to visualise.
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