Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12% PAGE)
Sample
African Green Monkey Cell lysate - whole cell (Vero cells)
Specification
Vero cells
Treatment
infected with rubella virus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Blotto
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Bands
Specific: 35 kDa
Exposure
30 second(s)
Negative control
Mock infected Vero cells
Positive control
RV infected Vero cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 10 2015