Anti-Robo2 + Robo3 抗体 (ab75014)
Key features and details
- Rabbit polyclonal to Robo2 + Robo3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Robo2 + Robo3 antibody -
製品の詳細
Rabbit polyclonal to Robo2 + Robo3 -
由来種
Rabbit -
特異性
Ab75014 has been shown to cross react with Robo2 and Robo3, but not Robo1. Robo3 expression is largely restricted to the developing cortex (PMID 30697732).
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Mouse Robo2 + Robo3 aa 1250-1350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab75013) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following tissue lysates: Rat Liver Mouse Liver
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75014の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 161 kDa).
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 161 kDa). |
ターゲット情報
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関連性
The function of the nervous system depends on the precision of axon wiring during development. SLITs, a family of secreted chemorepellent proteins, are crucial for the proper development of several major forebrain tracts. They act as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. ROBO2 (Roundabout, axon guidance receptor, homolog 2) is a receptor for SLIT2, and probably SLIT1 and mediates the function of SLITs. ROBO3 is thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity). Required for hindbrain axon midline crossing. -
細胞内局在
Membrane; Single-pass type I membrane protein. -
参照データベース
- Entrez Gene: 6092 Human
- Entrez Gene: 64221 Human
- Entrez Gene: 268902 Mouse
- Omim: 602431 Human
- SwissProt: Q96MS0 Human
- SwissProt: Q9HCK4 Human
- SwissProt: Q7TPD3 Mouse
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別名
- HGPPS 1 antibody
- HGPPS antibody
- HGPPS1 antibody
see all
画像
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All lanes : Anti-Robo2 + Robo3 antibody (ab75014) at 1/500 dilution
Lane 1 : Un-transfected HEK293
Lane 2 : HEK293 cells expressing Robo 1
Lane 3 : HEK293 cells expressing Robo 2
Lane 4 : HEK293 cells expressing Robo 3
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 161 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking: 2.5% Milk block for 1 hour at 20°C
Secondary incubation: TBS +2.5% Milk block for 16 hours at 4°C
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All lanes : Anti-Robo2 + Robo3 antibody (ab75014) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 161 kDa
Exposure time: 20 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab75014 は 4 報の論文で使用されています。
- Pinho AV et al. ROBO2 is a stroma suppressor gene in the pancreas and acts via TGF-ß signalling. Nat Commun 9:5083 (2018). PubMed: 30504844
- Xu R et al. Targeting skeletal endothelium to ameliorate bone loss. Nat Med 24:823-833 (2018). PubMed: 29785024
- Zeng Z et al. Slit2-Robo2 signaling modulates the fibrogenic activity and migration of hepatic stellate cells. Life Sci 203:39-47 (2018). PubMed: 29660433
- Rama N et al. Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization. Nat Med 21:483-91 (2015). PubMed: 25894826