Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T cells)
Loading amount
25 µg
Specification
HEK293T cells
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk-TBST
Secondary antibody
Name
Non-Abcam antibody was used: Li-Cor anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/15000
Detection
Detection method
Odyssey Infrared Imaging System
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 240 kDa
Positive control
this sample
Additional data
Additional Notes
A clear band for endogenous Robo2 was detected from a sample of HEK293T lysised in 1% Triton X-100-PBS.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 18 2013