Application
Western blot
Sample
Bird Tissue lysate - whole (Pigeon Retina, no boiling of sample prior to loadi)
Gel Running Conditions
Non-reduced Denaturing (7.5% Tris-Glycine)
Loading amount
20 µg
Specification
Pigeon Retina, no boiling of sample prior to loadi
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% milk in TBST (0.1% Tween-20)
Dilution
1/1000
Secondary antibody
Name
Abcam antibody:Goat F(ab) Anti-Mouse IgG H&L (HRP)
Detection
Detection method
ECL
Negative control
Not included
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 35 kDa
Positive control
Not included
Additional data
Additional Notes
WB membrane: Nitrocellulose
Lysate buffer: standard RIPA with protease inhibitor tablet
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Tobias Hochstöger
Verified customer
投稿 Sep 15 2017