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Product name
Anti-RhoA antibody
RhoA 抗体 (11件) 一覧
Description
Rabbit polyclonal to RhoA
Tested applications
WB, IHC-P, IHC-Fr, FPIA, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Orangutan
Immunogen
Synthetic peptide (Human), conjugated to an immunogenic carrier protein
Positive control
Spinal cord.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/300 - 1/3000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IHC-P: 1/300 - 1/3000. Antigen retrieval is recommended.
IHC-Fr: 1/300 - 1/3000.
FPIA: Use at an assay dependent concentration. PubMed: 21543326
ICC/IF: 1/200.
Target
Function
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.
Sequence similarities
Belongs to the small GTPase superfamily. Rho family.
Domain
The basic-rich region is essential for yopT recognition and cleavage.
Post-translational
modifications
Substrate for botulinum ADP-ribosyltransferase.
Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP61586
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Aplysia ras related homolog 12 antibody
- ARH 12 antibody
- ARH12 antibody
see all
Database links
- Entrez Gene: 395442 Chicken
- Entrez Gene: 338049 Cow
- Entrez Gene: 387 Human
- Entrez Gene: 11848 Mouse
- Entrez Gene: 117273 Rat
- Omim: 165390 Human
- SwissProt: O93467 Chicken
- SwissProt: P61585 Cow
see all
Anti-RhoA antibody images:
Western blot - RhoA antibody (ab68826)
Lane 1 : Anti-RhoA antibody (ab68826) at 1/2000 dilution
Lane 2 : Anti-RhoA antibody (ab68826) at 1/2000 dilution (pre-absorbed with immunizing peptide)
Lane 1 : Rat spinal cord lysate
Lane 2 : Rat spinal cord lysate
Predicted band size : 22 kDa
Observed band size : 22 kDa
Immunohistochemistry (Frozen sections) - RhoA antibody (ab68826)
ab68826 (1/500) staining RhoA (red) and ab72019 staining Sortilin (green) in rat trigeminal tissue. DAPI counterstaining appears in blue.
Immunocytochemistry/ Immunofluorescence - RhoA antibody (ab68826)
ICC/IF image of ab68826 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab68826 1/200 dilution overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-RhoA antibody (ab68826)
This product has been referenced in:
- Wu C et al. Rab13-dependent Trafficking of RhoA Is Required for Directional Migration and Angiogenesis. J Biol Chem 286:23511-20 (2011). FPIA; Mouse.Read more (PubMed: 21543326) »
- Wu YG et al. MPF governs the assembly and contraction of actomyosin rings by activating RhoA and MAPK during chemical-induced cytokinesis of goat oocytes. PLoS One 5:e12706 (2010). WB; Goat.Read more (PubMed: 20856880) »
See all 2 publications for this product
Publishing research using ab68826? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"