Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
Antigen retrieval step
Enzymatic
Sample
Rat Tissue sections (Gonad)
Specification
Gonad
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
This antibody Anti-RhoA antibody (ab54835), we used 1/200 dilution for both primary and secondary antibodies, that is working very well.
Protease-PBS solution (100mg protease/200ml PBS) at 37'C, 4mins used for antigen retrieval.
We can not submit the images for ab54835 in this review right-now, but we will update the reviews later
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 23 2013