Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Loading amount
50 µg
Specification
Glioblastoma cells
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: MMP in TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: SIGMA A3682
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/50000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 25 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Dec 04 2012