Application
Western blot
Sample
Rat Cell lysate - whole cell (C6 Rat glioma cell line and Rat primary Glia cells)
Loading amount
40 µg
Specification
C6 Rat glioma cell line and Rat primary Glia cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: IRDey 800CW Goat anti-Mouse IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Dilution
1/13000
Detection
Detection method
Odyssey imaging system
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 25kDa kDa Non-specific: 45kDa - only in rat kDa
Positive control
U87 - human glioblastoma cell line
Additional data
Additional Notes
Lane 1: Rat Primary Glia
Lane 2: Rat C6
Lane 3: Human U87
Lane 2: Rat C6
Lane 3: Human U87
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Revital Knirsh
Verified customer
投稿 Jun 24 2011