Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (lung epithelial cells)
Specification
lung epithelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% saponin
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/300
Incubation time
30 minute(s) · Temperature: 20°C · Diluent: 10% serum, 0.2% saponin in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor® 488 Goat Anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 31 2012