Application
Western blot
Sample
Mouse Cell lysate - whole cell (J774 cell line)
Loading amount
10 µg
Specification
J774 cell line
Treatment
co-culture with C. Albicans
Gel Running Conditions
Reduced Denaturing (14% gel)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% TBS milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL advance
Exposure
20 second(s)
Bands
Specific: 22 kDa Non-specific: 40 kDa
Positive control
GTP incubated cells for active Rho
Additional data
Additional Notes
lane 1 positive control (J774 cells incubated with GTP)
lane 2 J774 cells
lane 3 J774 cells + C Albicans
lane 2 J774 cells
lane 3 J774 cells + C Albicans
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 25 2009