Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, postnatal day 6
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/1000
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed
Additional data
Additional Notes
As a marker of stem cells, REX1 should be found in a subset of spermatogonia in the postnatal day 6 testis. We observed that this antibody outlines the testis cords, suggesting localization to peritubular myoid cells rather than spermatogonia.
Abcam response
Thank you for sharing this data with us. ab28141 has not previously been tested in IHC-Fr. Based on the data shown here, ab28141 is not suitable for this application, under these experimental conditions. We would welcome feedback from other researchers currently using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Bryan Niedenberger
Verified customer
投稿 Sep 15 2016