Application
Western blot
Sample
Mouse Cell lysate - whole cell (Murine Embryonic Stem Cell Lysate)
Loading amount
40 µg
Specification
Murine Embryonic Stem Cell Lysate
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% Blotto
Secondary antibody
Name
Non-Abcam antibody was used: Jackson
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 38 kDa Non-specific: 36, 34 kDa
Positive control
Murine Embryonic Stem Cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Utkarsh Kapoor
Verified customer
投稿 Dec 01 2011