Application
Western blot
Sample
Human Cell lysate - whole cell (293T, HepG2, MEF)
Loading amount
20 µg
Specification
293T, HepG2, MEF
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: HRP-conjugated anti-rabbit secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
5 minute(s) and 30 second(s)
Bands
Specific: 51 kDa Non-specific: 72, 40, 25 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Dongil Kim
Verified customer
投稿 Jan 21 2013