Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat cell lines)
Loading amount
20 µg
Specification
Rat cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA, PBS-Tween 0.1%
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti Rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Supersignal west dura
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 57, (35?) kDa
Additional data
Additional Notes
Order: (1) 9L, (2) PC12, (3) RIE-1
Strong reactivity to rat reptin (55 kDa). A fast migrating (35 kDa) species titrates with full-length reptin that could be a degradation product or an alternatively spliced form.
Strong reactivity to rat reptin (55 kDa). A fast migrating (35 kDa) species titrates with full-length reptin that could be a degradation product or an alternatively spliced form.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 22 2012