Anti-Rel B 抗体 [EP613Y] (ab33917)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP613Y] to Rel B
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Rel B antibody [EP613Y]
Rel B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP613Y] to Rel B -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Rel B aa 1-100 (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Raji and Daudi cell lysates. IHC-P: Human lymphoma tissue. Flow Cyt (intra): Daudi cells. IP: Raji cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP613Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33917の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/2000.
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WB |
1/500. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/50.
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特記事項 |
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Flow Cyt (Intra)
1/2000. |
WB
1/500. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/50. |
ターゲット情報
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機能
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. -
配列類似性
Contains 1 RHD (Rel-like) domain. -
ドメイン
Both N- and C-terminal domains are required for transcriptional activation. -
翻訳後修飾
Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton > centrosome. Co-localizes with NEK6 in the centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 5971 Human
- Omim: 604758 Human
- SwissProt: Q01201 Human
- Unigene: 654402 Human
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別名
- I REL antibody
- I-Rel antibody
- IREL antibody
see all
画像
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All lanes : Anti-Rel B antibody [EP613Y] (ab33917) at 1/500 dilution
Lane 1 : Raji cell lysate
Lane 2 : Daudi cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labeled.
Predicted band size: 62 kDa -
Immunohistochemical analysis of paraffin-embedded human lymphoma using ab33917
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling Rel B (red) with ab33917 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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Purified ab33917 at 1/50 dilution (2µg) immunoprecipitating Rel B in Raji whole cell lysate.
Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab33917 + Raji whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab33917 in Raji whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 62 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab33917 は 5 報の論文で使用されています。
- Wei Z et al. CircRFWD3 promotes HNSCC metastasis by modulating miR-27a/b/PPARγ signaling. Cell Death Discov 8:285 (2022). PubMed: 35690612
- Gu C et al. Dectin-1 Controls TSLP-Induced Th2 Response by Regulating STAT3, STAT6, and p50-RelB Activities in Dendritic Cells. Front Immunol 12:678036 (2021). PubMed: 34305908
- McMillan DH et al. Lung-targeted overexpression of the NF-?B member RelB inhibits cigarette smoke-induced inflammation. Am J Pathol 179:125-33 (2011). PubMed: 21703398
- Siak JJ et al. The nuclear-factor kappaB pathway is activated in pterygium. Invest Ophthalmol Vis Sci 52:230-6 (2011). PubMed: 20811049
- Huang Y et al. Gene expression profiling identifies emerging oncogenic pathways operating in extranodal NK/T-cell lymphoma, nasal type. Blood 115:1226-37 (2010). PubMed: 19965620