Recombinant rat DNA Polymerase beta protein (ab123225)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant rat DNA Polymerase beta protein
DNA Polymerase beta タンパク質・ペプチド 製品一覧 -
生理活性
Enzyme activity: 90 unit/µl (1unit of the enzyme activity incorporates 1 nanomole of dNTP into acid-insoluble fraction at 37°C in 60 min.)
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精製度
> 95 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Rat -
予測される分子量
38 kDa -
領域
2 to 335
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特性
Our Abpromise guarantee covers the use of ab123225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 6
Constituents: 2.28% Potassium chloride, 0.02% DTT, 0.79% Tris HCl, 0.003% EDTA, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- DNA directed DNA polymerase beta
- DNA pol beta
- DNA polymerase beta
see all -
機能
Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. -
配列類似性
Belongs to the DNA polymerase type-X family. -
ドメイン
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity. -
翻訳後修飾
Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. -
細胞内局在
Nucleus, Cytoplasm - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab123225 は 1 報の論文で使用されています。
- Date T et al. Expression of active rat DNA polymerase beta in Escherichia coli. Biochemistry 27:2983-90 (1988). PubMed: 3042024