Recombinant rat BVR protein (ab91622)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant rat BVR protein
BVR タンパク質・ペプチド 製品一覧 -
生理活性
BVR Assay: Positive (> 180 units/mg) 1 unit of BVR will transform 1 nanomole of biliverdin to bilirubin in an NADPH dependent reaction at pH 8.5 at 37°C in 1 minute.
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精製度
> 95 % SDS-PAGE.
Purified by Multi-Step Chromatography. > 95% pure as determined by SDS-PAGE and Western Blot analysis. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Rat
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特性
Our Abpromise guarantee covers the use of ab91622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
BVR Assay: Positive (> 180 units/mg) 1 unit of BVR will transform 1 nanomole of biliverdin to bilirubin in an NADPH dependent reaction at pH 8.5 at 37°C in 1 minute.
Protein previously labeled as Biliverdin Reductase.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.79% Tris HCl, 0.0292% EDTA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- BIEA_HUMAN
- Biliverdin IX alpha reductase
- Biliverdin reductase A
see all -
機能
Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor. -
組織特異性
Liver. -
パスウェイ
Porphyrin-containing compound metabolism; protoheme degradation. -
関連疾患
Hyperbiliverdinemia -
配列類似性
Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily. -
細胞内局在
Cytoplasm. - Information by UniProt
画像
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SDS-PAGE Analysis:
Lane 1: Molecular weight marker Lane 2: 0.5 µg ab91622
Lane 3: 1.0 µg ab91622 Lane 4: 2.0 µg ab91622
Lane 5: 5.0 µg ab91622 -
All lanes : BVR Polyclonal Antibody
Lane 1 : Molecular weight markers
Lane 2 : ab91622 at 0.1 µg -
BVR Activity: 1 µg of ab91622 was incubated with biliverdin in the presence of NADPH at pH 8.5, 37°C. Product formation was monitored with a spectrometer at 450 nm.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab91622 は論文での使用が確認できていません。