Recombinant mouse Urokinase protein (ab92641)
Key features and details
- Expression system: Insect cells
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: ELISA, SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant mouse Urokinase protein
Urokinase タンパク質・ペプチド 製品一覧 -
生理活性
The uPA activity of this product was tested using urokinase chromogenic substrate and standardized to the WHO 2nd International Standard for Human High Molecular Weight Urokinase, NIBSC 11/184. If you need the specific activity value please contact us with the lot# you require the data for.
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精製度
> 98 % SDS-PAGE. -
発現系
Insect cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
配列
GSVLGAPDES NCGCQNGGVC VSYKYFSRIR RCSCPRKFQG EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV LQKPYNAHRP DAISLGLGKH NYCRNPDNQK RPWCYVQIGL RQFVQECMVH DCSLSKKPSS SVDQQGFQCG QKALRPRFKI VGGEFTEVEN QPWFAAIYQK NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY LGQSKESSYN PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP HYYGSEINYK MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR GCAEKNKPGV YTRVSHFLDW IQSHIGEEKG LAF -
予測される分子量
44 kDa -
領域
21 to 433 -
配列の追加情報
(Gene ID: 18792) Full length mature protein, without the signal peptide.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab92641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
ELISA
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
Spectrophotometric Data:
Ultraviolet Absorbance (280nm) = 0.9
epsilon 0.1% = 1.48
ab92641 demonstrates preferential binding to mouse uPAR over Human uPAR coated on an ELISA plate.
Produced using non-baculovirus insect cells.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 5.00
Constituents: 0.41% Sodium acetate, 0.0292% EDTA, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- ATF
- ATF uPA
- BDPLT5
see all -
機能
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
組織特異性
Expressed in the prostate gland and prostate cancers. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
翻訳後修飾
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
細胞内局在
Secreted. - Information by UniProt
画像
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SDS-PAGE analysis of mouse urokinase protein (ab92641) under reducing and non-reducing conditions in 12% SDS-PAGE. The urokinase protein is demonstrated to be active through the observed binding to Recombinant human PAI1 (mutated N150H + K154T + Q319L + M354I) protein (ab92969) (CPAI protein). CPAI is an inhibitor of urokinase, mutated to be more stable, and the two form a complex that migrates in the gel to a higher molecular weight.
The observed band at 44 kDa in lane 1 corresponds to mouse Urokinase protein under non-reducing conditions.
Lane 1: 3 µg mouse urokinase protein, non-reduced.
Lane 2: + CPAI, 10 µg, non-reduced.Lane 3: Molecular Weight Markers
Lane 4: 3 µg mouse urokinase protein, reduced.
Lane 5: + CPAI, 10 µg, reduced. -
SDS-PAGE analysis of mouse urokinase protein (ab92641) under reducing and non-reducing conditions in 12% SDS-PAGE. The urokinase protein is demonstrated to be active through the observed binding to Recombinant human PAI1 (mutated N150H + K154T + Q319L + M354I) protein (ab92969) (CPAI protein). CPAI is an inhibitor of urokinase, mutated to be more stable, and the two form a complex that migrates in the gel to a high molecular weight.
The observed band at 44 kDa in lane 3 corresponds to mouse Urokinase protein under non-reducing conditions.
Lane 1: 5 µg mouse urokinase protein, reduced.
Lane 2: + CPAI, 20 ug, reduced.
Lane 3: 5 ug mouse urokinase protein, non-reduced.
Lane 4: + CPAI, 20 ug, non-reduced.
Lane 5: Prestained Standard Ladder
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab92641 は 1 報の論文で使用されています。
- Semina EV et al. Downregulation of uPAR promotes urokinase translocation into the nucleus and epithelial to mesenchymal transition in neuroblastoma. J Cell Physiol 235:6268-6286 (2020). PubMed: 31990070