製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Mouse
    • 分子量
      18 kDa
    • 領域
      26 to 190
    • 配列の追加情報
      Murine SCF is a 18.3 kDa protein containing 164 amino acid residues, as the soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.

関連製品

特性

Our Abpromise guarantee covers the use of ab106309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    The ED50 as determined by the dose-dependent stimulation of the proliferation of the human TF-1 cell line is < 10 ng/ml, corresponding to a specific activity of >1 x 105 units/mg.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • エンドトキシン・レベル
    < 0.100 Eu/µg
  • 精製度
    > 98 % SDS-PAGE.

  • 製品の状態
    Lyophilised
  • 備考

    Endotoxin level: < 0.1 ng/g of SCF

     

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 30mM Tris, 100mM Sodium chloride, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Reconstitute in 50 mM acetic acid or water to a concentration of 0.1 mg/ml. Do not vortex

関連情報

  • 別名
    • C kit ligand
    • C-kit ligand
    • Ckit ligand
    • DCUA
    • DFNA69
    • DKFZp686F2250
    • familial progressive hyperpigmentation 2
    • FPH2
    • FPHH
    • KIT ligand
    • Kitl
    • KITLG
    • KL 1
    • KL1
    • Mast cell growth factor
    • MGF
    • MGF stem cell factor
    • SCF
    • SCF_HUMAN
    • SF
    • SHEP7
    • sKITLG
    • Soluble KIT ligand
    • Steel factor
    • steel, mouse, homolog of
    • Stem cell factor
    • Stem cell factor precursor
    see all
  • 機能
    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • 関連疾患
    Hyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • 配列類似性
    Belongs to the SCF family.
  • 発生段階
    Acts in the early stages of hematopoiesis.
  • 翻訳後修飾
    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • 細胞内局在
    Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt

画像

  • 15% SDS-PAGE.
    Lane 1: Molecular weight marker.
    Lane 2: SCF protein.
    Visualised by silver stain.

参考文献

ab106309 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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