Recombinant mouse IL-31 protein (ab119155)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC
製品の詳細
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製品名
Recombinant mouse IL-31 protein
IL-31 タンパク質・ペプチド 製品一覧 -
生理活性
The activity is determined by the dose dependent production of IL-8 from human PBMCs and is typically less than 25 ng/mL. -
精製度
> 95 % SDS-PAGE.
Protein content and purity of ab119155 was determined by UV spectroscopy at 280 nm, RP-HPLC calibrated against a known standard and quantitation against a known standard via reducing and non-reducing SDS-PAGE gels. Purified using standard chromatographical techniques. -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
配列
MTCSLSFGAPISKEDLRTTIDLLKQESQDLYNNYSIKQASGMSADESIQL PCFSLDREALTNISVIIAHLEKVKVLSENTVDTSWVIRWLTNISCFNPLN LNISVPGNTDESYDCKVFVLTVLKQFSNCMAELQAKDNTTC -
予測される分子量
16 kDa -
領域
24 to 163
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特性
Our Abpromise guarantee covers the use of ab119155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
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製品の状態
Lyophilized -
備考
It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituent: 0.14% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Lyophilized product is very stable at -20°C. Centrifuge vial before opening. When reconstituting, gently pipette and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute ab119155 with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
関連情報
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別名
- IL 31
- IL-31
- IL31
see all -
機能
Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. -
組織特異性
Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. -
細胞内局在
Secreted. - Information by UniProt
画像
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SDS PAGE analysis of ab119155 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
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ab119155 used in Western Blot. Figure: 1 ug run under (+) reducing conditions and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Mouse IL-31 has a predicted MW of 15.7 kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab119155 は論文での使用が確認できていません。