Application
Western blot
25 ng of total Timp1 protein loaded
PAGE 12% reducing-denaturing
Blocked in 10% milk for 18 hrs at 4oC
primary antibody- rabbit anti Timp1
Secondary antibody- biotinylated anti-rabbit
detection ABC system
PAGE 12% reducing-denaturing
Blocked in 10% milk for 18 hrs at 4oC
primary antibody- rabbit anti Timp1
Secondary antibody- biotinylated anti-rabbit
detection ABC system
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Henda Nabli
Verified customer
投稿 Oct 18 2013