Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (ab50232)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, WB
製品の詳細
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製品名
Recombinant human Serum Amyloid A (mutated D60N + H71R) protein
Serum Amyloid A タンパク質・ペプチド 製品一覧 -
生理活性
Tested by its ability to down- regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4 μM. Schreiber, BM. et al. Biochem J. 1999 Nov 15; 344 Pt 1:7-13
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精製度
>= 98 % SDS-PAGE.
>=98% by SDS-PAGE and HPLC analyses. -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS NARENIQRFF GRGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY -
予測される分子量
12 kDa -
修飾
mutated D60N + H71R
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特性
Our Abpromise guarantee covers the use of ab50232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
Western blot
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製品の状態
Lyophilized -
備考
For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50 µg/ml. ab50232 can be used as a WB positive control in conjunction with ab687.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Please reconstitute this product in water to a concentration of 1mg/ml.
関連情報
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別名
- Amyloid fibrils
- SAA 2
- Serum amyloid A1 isoform 2
see all -
関連性
Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
画像
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Anti-Serum Amyloid A antibody [115] (ab687) at 0.5 µg/ml +
Recombinant human Serum Amyloid A (mutated D60N + H71R) protein (ab50232) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab50232 は論文での使用が確認できていません。