Recombinant human Peroxiredoxin 1/PAG protein (ab74172)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Peroxiredoxin 1/PAG protein
Peroxiredoxin 1/PAG タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity: approximately 600-670 pmole/min/µg. Enzymatic activity is defined as the amount of hydroperoxide that 1µg of enzyme can reduce at 25C for 1 minute. Conditions - Reaction buffer : 1mM DTT, 0.03X PBS, 0.5% glycerol. - Initial amount of substrate : 0.85µg - Total reaction volume: 100 µl. - Reaction temperature: Room temperature Specific activity: approximately 600-670 pmole/min/µg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX1 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute.
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精製度
> 90 % SDS-PAGE.
ab74172 is purified by conventional chromatography techniques. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MSSGNAKIGH PAPNFKATAV MPDGQFKDIS LSDYKGKYVV FFFYPLDFTF VCPTEIIAFS DRAEEFKKLN CQVIGASVDS HFCHLAWVNT PKKQGGLGPM NIPLVSDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITVNDLPVG RSVDETLRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVQK SKEYFSKQK -
タグ
His tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab74172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.242% Tris, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Heme binding 23 kDa protein
- MSP23
- Natural killer cell-enhancing factor A
see all -
機能
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. -
配列類似性
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
翻訳後修飾
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. -
細胞内局在
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab74172 は 2 報の論文で使用されています。
- Li P et al. LIF, a mitogen for choroidal endothelial cells, protects the choriocapillaris: implications for prevention of geographic atrophy. EMBO Mol Med 14:e14511 (2022). PubMed: 34779136
- Chung MC et al. S-nitrosylation of peroxiredoxin 1 contributes to viability of lung epithelial cells during Bacillus anthracis infection. Biochim Biophys Acta Gen Subj 1861:3019-3029 (2017). PubMed: 27612662