Recombinant human PDE10A protein (ab101116)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human PDE10A protein
PDE10A タンパク質・ペプチド 製品一覧 -
生理活性
The specific activity of ab101116 was determined to be 92 nmol/min/mg as per activity assay.
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精製度
> 80 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected insect cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MQGVVYELNSYIEQRLDTGGDNQLLLYELSSIIKIATKADGFALYFLGEC NNSLCIFTPPGIKEGKPRLIPAGPITQGTTVSAYVAKSRKTLLVEDILGD ERFPRGTGLESGTRIQSVLCLPIVTAIGDLIGILELYRHWGKEAFCLSHQ EVATANLAWASVAIHQVQVCRGLAKQTELNDFLLDVSKTYFDNIVAIDSL LEHIMIYAKNLVNADRCALFQVDHKNKELYSDLFDIGEEKEGKPVFKKTK EIRFSIEKGIAGQVARTGEVLNIPDAYADPRFNREVDLYTGYTTRNILCM PIVSRGSVIGVVQMVNKISGSAFSKTDENNFKMFAVFCALALHCANMYHR IRHSECIYRVTMEKLSYHSICTSEEWQGLMQFTLPVRLCKEIELFHFDIG PFENMWPGIFVYMVHRSCGTSCFELEKLCRFIMSVKKNYRRVPYHNWKHA VTVAHCMYAILQNNHTLFTDLERKGLLIACLCHDLDHRGFSNSYLQKFDH PLAALYSTSTMEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAI IATDLALYFGNRKQLEEMYQTGSLNLNNQSHRDRVIGLMMTACDLCSVTK LWPVTKLTANDIYAEFWAEGDEMKKLGIQPIPMMDRDKKDEVPQGQLGFY NAVAIPCYTTLTQILPPTEPLLKACRDNLSQWEKVIRGEETATWISSPSV AQKAAASED -
予測される分子量
102 kDa including tags -
領域
71 to 779
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特性
Our Abpromise guarantee covers the use of ab101116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- 5''-cyclic phosphodiesterase 10A
- cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A
- cAMP and cAMP-inhibited cGMP 3''
see all -
機能
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. -
組織特異性
Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum. -
パスウェイ
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. -
配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
ドメイン
The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region. -
細胞内局在
Cytoplasm. Located mostly to soluble cellular fractions. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab101116 は論文での使用が確認できていません。