Recombinant human MSK1 protein (ab84805)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB, Functional Studies
製品の詳細
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製品名
Recombinant human MSK1 protein -
生理活性
The Specific activity of ab84805 was determined to be 89 nmol/min/mg.
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精製度
> 90 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
タグ
GST tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab84805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
Functional Studies
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製品の状態
Liquid -
備考
ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- 90 kDa ribosomal protein S6 kinase 5
- EC 2.7.11.1
- KS6A5_HUMAN
see all -
機能
Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). -
組織特異性
Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻訳後修飾
Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. -
細胞内局在
Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic. - Information by UniProt
画像
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The specific activity of MSK1 (ab84805) was determined to be 9.9 nmol/min/mg as per activity assay protocol and was equivalent to 75 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab84805
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SDS PAGE analysis of ab84805
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The Specific activity of ab84805 was determined to be 89 nmol/min/mg.
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SDS-PAGE showing ab84805 at approximately 120kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab84805 は論文での使用が確認できていません。