Recombinant human IL-12 protein (ab89013)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human IL-12 protein
IL-12 タンパク質・ペプチド 製品一覧 -
生理活性
Activity was measured by its ability to stimulate the proliferation of PHA-activated human T-lymphoblasts, and is typically in the range of 0.05-0.20 ng/ml.
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精製度
> 95 % SDS-PAGE. -
発現系
HEK 293 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
Theoretical Sequence Alpha Chain: RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPC TSEEIDHE DITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLA SRKTSFMMAL CLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNML AVIDELMQALNF NSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVT IDRVMSYLNAS Beta Chain: IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQ SSEVLGSG KTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIW STDILKDQKE PKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSR GSSDPQGVTCGA ATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPI EVMVDAVHKLKYEN YTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSW EYPDTWSTPHSYFSLT FCVQVQGKSKREKKDRVFTDKTSATVICRKNA SISVRAQDRYYSSSWSEW ASVPCS
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab89013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 1% Human serum albumin, 10% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
関連情報
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別名
- CLMF
- CLMF p35
- CLMF p40
see all -
機能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
配列類似性
Belongs to the IL-6 superfamily. -
細胞内局在
Secreted. - Information by UniProt
画像
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1D SDS-PAGE of ab89013 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab89013
Lane 2: ab89013 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab89013 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Appearance of additional band at lower MWt after treatment with PNGase F indicates the presence of N-linked glycans. The subsequent drop in MWt after treatment with a glycosidase cocktail indicates the presence of O-linked glycans. Additional high MWt bands in lane 3 are glycosidase enzymes. -
A sample of ab89013 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg of protein was loaded. Spot trains indicate presence of multiple isoforms.
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Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangles indicate theoretical pI and MWt of the alpha and beta chains of the protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab89013 は論文での使用が確認できていません。