Recombinant human HDAC3 protein (ab101663)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human HDAC3 protein
HDAC3 タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity 125 RLU/min/ng.
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精製度
> 70 % SDS-PAGE.
ab101663 is >70% pure as assessed by densitometry. Affinity purified. -
発現系
Baculovirus infected insect cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
83 kDa including tags -
領域
1 to 428
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab101663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- HD3
- HDAC 3
- HDAC3
see all -
機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻訳後修飾
Sumoylated in vitro. -
細胞内局在
Nucleus. - Information by UniProt
画像
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The specific activity of HDAC3 (ab101663) was determined to be 144 RLU/min/mg as per activity assay protocol
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SDS PAGE analysis of ab101663
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SDS PAGE analysis of ab101663
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The specific activity of ab101663 was determined to be 125 RLU/min/ng.
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The purity of HDAC3 was determined to be >70% as assessed by densitometry.
The approximate molecular weight is ~83 kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab101663 は論文での使用が確認できていません。