Recombinant human MAP4K3/GLK protein (Tagged) (ab177265)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human MAP4K3/GLK protein (Tagged) -
生理活性
92 nmol/min/mg
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精製度
> 70 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLE PGEDFAVVQQEIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQD IYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHV KLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAV GITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFV KMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDF DDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHH ELPDSDGFLD SSEEIYYTARSNLDLQLEYG -
予測される分子量
70 kDa including tags -
領域
1 to 380 -
タグ
GST tag N-Terminus
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関連製品
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Related Products
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab177265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
ab204848 (c Abl peptide) can be utilized as a substrate for assessing Kinase activity
Previously labelled as MAP4K3.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.005% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Germinal center kinase like kinase
- Germinal center kinase-related protein kinase
- GLK
see all -
関連性
May play a role in the response to environmental stress. This gene encodes a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. -
細胞内局在
Cytoplasmic and Nuclear
画像
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The specific activity of MAP4K3/GLK (ab177265) was determined to be 39.1 nmol/min/mg as per activity assay protocol and was equivalent to 91.9 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab177265
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SDS PAGE analysis of ab177265
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The specific activity of ab177265 was determined to be 92 nmol/min/mg.
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SDS-PAGE analysis of ab177265.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab177265 は論文での使用が確認できていません。