Recombinant human ANKK1 protein (ab204164)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human ANKK1 protein -
生理活性
The Specific activity of ab204164 was determined to be 80 nmol/min/mg.
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精製度
> 75 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MAADPTELRLGSLPVFTRDDFEGDWRLVASGGFSQVFQARHRRWRTEYAI KCAPCLPPDAASSDVNYLIEEAAKMKKIKFQHIVSIYGVCKQPLGIVMEF MANGSLEKVLSTHSLCWKLRFRIIHETSLAMNFLHSIKPPLLHLDLKPGN ILLDSNMHVKISDFGLSKWMEQSTRMQYIERSALRGMLSYIPPEMFLESN KAPGPKYDVYSFAIVIWELLTQKKPYSGFNMMMIIIRVAA GMRPSLQP VSDQWPSEAQQMVDLMKRCWDQDPKKRPCFLDITIETDILLSLLQSRVAV PESKALARKVSCKLSLRQPGEVNEDISQELMDSDSGNYLKRALQLSDRKN LVPRDEELCIYENKVTPLHFLVAQGSVEQVRLLLAHEVDVDCQTASGYTP LLIAAQDQQPDLCALLLAHGADANRVDEDGWAPLHFAAQNGDDGTARLLL DHGACVDAQEREGWTPLHLAAQNNFENVARLLVSRQADPNLHEAEGKTPL HVAAYFGHVSLVKLLTSQGAELDAQQRNLRTPLHLAVERGKVRAIQHLLK SGAVPDALDQSGYGPLHTAAARGKYLICKMLLRYGASLELPTHQGWTPLH LAAYKGHLEIIHLLAESHANMGALGAVNWTPLHLAARHGEEAVVSALLQC GADPNAAEQSGWTPLHLAVQRSTFLSVINLLEHHANVHARNKVGWTPAHL AALKGNTAILKVLVEAGAQLDVQDGVSCTPLQLALRSRKQGIMSFLEGKE PSVATLGGSKPGAEMEI -
予測される分子量
115 kDa including tags -
領域
1 to 765 -
タグ
GST tag N-Terminus -
配列の追加情報
NM_178510.
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関連製品
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab204164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.002% PMSF, 0.004% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Ankyrin repeat and kinase domain containing 1
- Ankyrin repeat and protein kinase domain containing protein 1
- EC 2.7.11.1
see all -
関連性
ANKK1 belongs to the TKL Ser/Thr protein kinase superfamily, contains twelve ANK repeats and one protein kinase domain. The protein is weakly expressed in testis, placenta, and spinal cord.
画像
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The specific activity of ANKK1 (ab204164) was determined to be 1 nmol/min/mg as per activity assay protocol and was equivalent to 7 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab204164
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SDS PAGE analysis of ab204164
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Analysis of the purity of ab204164 by densitometry.
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Kinase assay demonstrating specific activity of ab204164.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab204164 は論文での使用が確認できていません。