Recombinant thermus aquaticus RecA protein (ab123228)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, Electron Microscopy
製品の詳細
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製品名
Recombinant thermus aquaticus RecA protein
RecA タンパク質・ペプチド 製品一覧 -
生理活性
The activity of single-stranded DNA-dependent ATPase was confirmed.
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精製度
> 90 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Thermus aquaticus -
予測される分子量
37 kDa -
領域
1 to 340 -
配列の追加情報
This Protein is from Thermus aquaticus
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特性
Our Abpromise guarantee covers the use of ab123228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
Electron Microscopy
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C.
pH: 6.5
Constituents: 0.79% Tris HCl, 0.03% EDTA, 50% Glycerol (glycerin, glycerine), 1.17% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- DNA strand exchange and recombination protein with protease and nuclease activity
- lexB
- Protein recA
see all -
関連性
RecA in E.coli can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage. -
細胞内局在
Cytoplasmic
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab123228 は 2 報の論文で使用されています。
- Shigemori Y et al. Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products. Nucleic Acids Res 33:e126 (2005). PubMed: 16087733
- Angov E & Camerini-Otero RD The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization. J Bacteriol 176:1405-12 (1994). PubMed: 8113181