Recombinant JCV Polyomavirus Major Capsid VP1 protein (ab74569)
Key features and details
- Expression system: Saccharomyces cerevisiae
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE
製品の詳細
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製品名
Recombinant JCV Polyomavirus Major Capsid VP1 protein
JCV Polyomavirus Major Capsid VP1 タンパク質・ペプチド 製品一覧 -
生理活性
JCV VP1 protein was purified and lyophilized assembled into virus like particles (VLPs). It is tested for hemagglutination activity and analysed using electron microscopy.
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精製度
> 95 % SDS-PAGE.
Purified by ultracentifugation. -
発現系
Saccharomyces cerevisiae -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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配列
MAPTKRKGERKDPVQVPKLLIRGGVEVLEVKTGVDSITEVECFLTPEMGD PDEHLRGFSKSISISDTFESDSPNKDMLPCYSVARIPLPNLNEDLTCGNI LMWEAVTLKTEVLGVTTLMNVHSNGQATHDNGAGKPVQGTSFHFFSVGGE ALELQGVVFNYRTKYPDGTIFPKNATVQSQVMNTEHKAYLDKNKAYPVEC WVPDPTRNENTRYFGTLTGGENVPPVLHITNTATTVLLDEFGVGPLCKGD NLYLSAVDVCGMFTNRSGSQQWRGLSRYFKVQLRKRRVKNPYPISFLLTD LINRRTPKVDGQPMYGMDAQIEEVRVFEGTEELPGDPDMMRYVDRYGQLQ TKML -
予測される分子量
40 kDa -
実際の分子量
41 kDa -
領域
1 to 354
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab74569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
SDS-PAGE
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製品の状態
Lyophilized -
備考
JCV VP1 protein was purified and lyophilized assembled into virus like particles (VLPs). It is tested for hemagglutination activity and analysed using electron microscopy.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C.
Constituent: PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with deionized H2O. After reconstitution store at 4°C. With the addition of glycerol store at -20°C.
関連情報
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別名
- Capsid protein VP1
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関連性
The human polyomavirus JC virus (JCV) infects greater than 80% of the human population. The JC virus is a small (38-40 nm in diameter) double stranded, circular DNA virus covered by an icosahedral capsid. Infection with JCV is asymptomatic and it occurs in early childhood. After the primary infection, the virus remains in latent state in the kidney, until it's reactivation under immunosuppressive conditions to result in Progressive Multifocal Leukoencephalopathy (PML), a fatal demyelinating disease. 70% of all HIV-1- infected patients will exhibit neurological disorders and between 5 and 8% of all HIV-1-infected patients will develop PML. Similar to other polyomaviruses, JCV can cause tumors when intracerebrally inoculated at high titers into developing rodent. Several reports suggest the association of viruses, especially of the polyomavirus family with different types of human brain tumors. Tumorigenecity of JCV is most likely induced by the viral early gene product T-antigen. T-antigen has the capacity to interact with several tumor suppressor proteins, most notably p53, and functionally inactivate these proteins. -
細胞内局在
Virion. Nucleus
画像
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Recombinant JCV Polyomavirus Major Capsid VP1 protein (ab74569)
Predicted band size: 40 kDaWestern blot showing ab74569 (Lane 1)
MW ladder (Lane 2)
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SDS-PAGE showing ab74569 (Lane 1)
Lane 2 reprents the molecular weight ladder. -
SDS-PAGE showing ab74569 (4µg/lane).
Lane 1 reprents the molecular weight ladder. From the bottom: 14.4, 18.4, 25.0, 35.0, 45.0, 66.2 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab74569 は 3 報の論文で使用されています。
- López-Moncada F et al. Nucleolar Expression and Chromosomal Associations in Robertsonian Spermatocytes of Mus musculus domesticus. Genes (Basel) 10:N/A (2019). PubMed: 30736350
- Tan CS et al. Brief Report: Decreased JC Virus-Specific Antibody-Dependent Cellular Cytotoxicity in HIV-Seropositive PML Survivors. J Acquir Immune Defic Syndr 82:220-224 (2019). PubMed: 31513076
- Saccardo P et al. Effect of the DnaK chaperone on the conformational quality of JCV VP1 virus-like particles produced in Escherichia coli. Biotechnol Prog 30:744-8 (2014). PubMed: 24574306