Recombinant Human CTNNA1 protein (Tagged) (ab51443)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: = 95% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB
製品の詳細
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製品名
Recombinant Human CTNNA1 protein (Tagged) -
精製度
= 95 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
123 kDa including tags -
タグ
GST tag N-Terminus -
配列の追加情報
NM 001903
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特性
Our Abpromise guarantee covers the use of ab51443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
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製品の状態
Liquid -
備考
This product was previously labelled as alpha 1 Catenin
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
関連情報
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別名
- Alpha E-catenin
- Cadherin associated protein
- Cadherin associated protein 102kDa
see all -
機能
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. -
組織特異性
Expressed ubiquitously in normal tissues. -
配列類似性
Belongs to the vinculin/alpha-catenin family. -
翻訳後修飾
Sumoylated. -
細胞内局在
Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab51443 は 1 報の論文で使用されています。
- Yosefzon Y et al. Caspase-3 Regulates YAP-Dependent Cell Proliferation and Organ Size. Mol Cell 70:573-587.e4 (2018). Functional Studies . PubMed: 29775577