Recombinant Human Hsp60 protein (ab113177)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 50.000 Eu/mg
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant Human Hsp60 protein
Hsp60 タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
ab113177 was purified by multi-step chromatography. -
エンドトキシン・レベル
< 50.000 Eu/mg -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
61 kDa -
領域
1 to 573
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab113177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 99% PBS, Phosphate Buffer
関連情報
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別名
- 60 kDa chaperonin
- 60 kDa heat shock protein, mitochondrial
- CH60_HUMAN
see all -
機能
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
関連疾患
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
配列類似性
Belongs to the chaperonin (HSP60) family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
画像
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SDS-PAGE analysis of ab113177: Lane 1: MW marker, Lane 2: 0.5ug, Lane 3: 1ug, Lane 4: 2.5ug, Lane 5: 5ug, Lane 6: 10ug.
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All lanes : a monoclonal anti Hsp60 protein
Lane 1 : molecualr weight marker
Lane 2 :Recombinant Human Hsp60 protein (ab113177) at 0.1 µg
Lane 3 :Recombinant Human Hsp60 protein (ab113177) at 0.05 µg
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab113177 は 1 報の論文で使用されています。
- Shi L et al. Anti-heat shock protein autoantibody profiling in breast cancer using customized protein microarray. Anal Bioanal Chem 408:1497-506 (2016). PubMed: 26715250