Recombinant Human Ghrelin protein (ab89233)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: WB, SDS-PAGE
製品の詳細
-
製品名
Recombinant Human Ghrelin protein -
精製度
> 90 % SDS-PAGE.
ab89233 is purified using conventional chromatography techniques. Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method). -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
タグ
His tag N-Terminus
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab89233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Western blot
SDS-PAGE
-
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 1.12% Sodium chloride, 1.74% PMSF
関連情報
-
別名
- Appetite regulating hormone
- Ghrelin
- Ghrelin 27
see all -
機能
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility. -
組織特異性
Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy. -
配列類似性
Belongs to the motilin family. -
翻訳後修飾
O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
Amidation of Leu-98 is essential for obestatin activity. -
細胞内局在
Secreted. - Information by UniProt
画像
-
15% SDS-PAGE showing ab89233 (3µg).
Note: Real molecular weight on SDS-PAGE is shifted up compared to predicted molecular weight.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab89233 は 1 報の論文で使用されています。
- Oiso S et al. Establishment of a Gastric Cell-Based Assay System for Exploring Inhibitors of Octanoylated Ghrelin Production. J Biomol Screen N/A:N/A (2013). PubMed: 23704134