Recombinant Human CD134 / OX40L receptor protein (Fc Chimera) (ab83676)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Human CD134 / OX40L receptor protein (Fc Chimera)
CD134 / OX40L receptor タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
Theoretical sequence: LHCVGDTYPSNDRCCHECRPGNGMVSRCSRSQNTVCRPC GPGFYNDVVSSKPCKP CTWCNLRSGSERKQLCTATQDTVCRCRAGTQP LDSYKPGVDCAPCPPGHFSPGDNQ ACKPWTNCTLAGKHTLQPASNSSD AICEDRDPPATQPQETQGPPARPITVQPTEAWP RTSQGPSTRPVGIPK VDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMIS RTPE VTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTV LHQ DWLNGKEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRD ELTKNQVSLTCLV KGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSF FLYSKLTVDKSRWQQGNVFSCS VMHEALHNHYTQKSLSLSPGK -
領域
1 to 207 -
配列の追加情報
Fused with the Fc region of Human IgG1 at the C-terminus.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab83676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: PBS, 1% Human serum albumin, 10% Trehalose
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再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
関連情報
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別名
- ACT 35
- ACT35
- ACT35 antigen
see all -
機能
Receptor for TNFSF4/OX40L/GP34. -
配列類似性
Contains 4 TNFR-Cys repeats. -
細胞内局在
Membrane. - Information by UniProt
画像
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Lane 1 MW markers; Lane 2 ab83676; Lane 3 ab83676 treated with PNGase F to remove potential N-linked glycans; Lane 4 ab83676 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 μg protein loaded per lane; Deep Purple™ stained.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Slight drop in MW after treatment with glycosidase cocktail suggests presence of Olinked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes. -
A sample of ab83676 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris HCl 2D gel. 40 μg protein loaded per lane; Deep Purple™ stained. Spot train indicates presence of multiple isoforms of the Chimera. Spots within the spot train were cut from the gel and identified as the Chimera by protein mass fingerprinting.
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Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified ab83676 exists in multiple isoforms, which differ according to their level of post-translational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins.
Triangle indicates theoretical pI and MW of the protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab83676 は論文での使用が確認できていません。