Recombinant Human Bmi1 protein (ab114169)
Key features and details
- Expression system: Wheat germ
- Suitable for: WB, ELISA, SDS-PAGE
製品の詳細
-
製品名
Recombinant Human Bmi1 protein
Bmi1 タンパク質・ペプチド 製品一覧 -
発現系
Wheat germ -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETS KYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAA HPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKD KEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPL KDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELE SDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSP SGNHQSSFANRPRKASVNGSSATSSG -
予測される分子量
62 kDa including tags -
領域
1 to 326
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab114169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Western blot
ELISA
SDS-PAGE
-
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.3% Glutathione, 0.79% Tris HCl
関連情報
-
別名
- B lymphoma Mo MLV insertion region (mouse)
- B lymphoma Mo MLV insertion region 1 homolog
- Bmi 1
see all -
機能
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. -
配列類似性
Contains 1 RING-type zinc finger. -
翻訳後修飾
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
画像
-
ab114169 on a 12.5% SDS-PAGE Stained with Coomassie Blue.
-
Anti-Bmi1 antibody (ab63767) at 2 µg/ml +
Recombinant Human Bmi1 protein (ab114169) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 secondsab63767 recognizes the full length tagged recombinant Bmi1 protein (ab114169) which has an expected molecular weight of 62 kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab114169 は論文での使用が確認できていません。