Recombinant E. coli slyD protein (ab72847)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant E. coli slyD protein -
生理活性
Specific activity is > 700nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmol of suc-AAPF-pNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin.
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精製度
> 95 % SDS-PAGE.
ab72847 is purified by conventional chromatography. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Escherichia coli -
配列
MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISGLETALE GHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAET DQGPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHG HVHGAHDHHHDHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH
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特性
Our Abpromise guarantee covers the use of ab72847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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質量分析
MALDI-TOF -
製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituent: 0.242% TrisThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- FKBP type peptidyl prolyl cis trans isomerase slyD
- Histidine rich protein
- PPIase
see all -
関連性
slyD belongs to the FKBP-type PPIase family.Required for lysis of phiX174 infected cells. It binds nickel and zinc with high affinity and 1:1 stoichiometry, copper and cobalt with lower affinity. No binding is detectable for ferrous, ferric, magnesium and calcium ions. The activity of slyD is considerably smaller than the one found in other PPIases with the same substrate. The substrate specificity carried out with 'suc-Ala-Xaa-Pro-Phe-4NA', where Xaa is the AA tested, was found to be Phe > Ala > Leu. -
細胞内局在
Cytoplasmic
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab72847 は論文での使用が確認できていません。