Sample: Rat Tissue lysate - whole (spinal cord)
Specification: spinal cord
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: Traumatized segment level T13
Did you use the Abcam Kit protocol?: Yes
Additional data
Additional Notes: I have used the kit with fresh rat spinal cord samples that underwent compression. The samples where homogenized a day before and kept in -20c. The strips allowed me to use the kit 3 times. The third time was a week after I have open Item F and still got a nice curve. R-square=0.9959.
Results are more than satisfying. It is a quick and straight forward assay.
Graph labels: Y axis is absorbance, X axis is pg/ml.The key labels are, from top to bottom: Standards, suppresses stds, standard curve.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Prof. Guy Sovak
Verified customer
投稿 May 21 2012