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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (FITC)
IgG 抗体 (1691件) 一覧
Target species
Rat
Tested applications
Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
Immunogen
Rat whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Rat >> IgG >> Fluorophore >> FITC
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (FITC) (ab6730)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab6730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
- 1 Image
IHC-Fr
ELISA
Application notes
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (FITC) images:
Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (FITC) (ab6730)
Ab6730 was used at dilution 1/500 with the primary antibody ab53444 in IHC-Fr. See the review on ab53444.
References for Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (FITC) (ab6730)
This product has been referenced in:
- Bogomolnaya LMet al. A new enrichment approach identifies genes that alter cell cycle progression in Saccharomyces cerevisiae. Curr Genet 45:350-9 (2004).Read more (PubMed: 15022016) »
See 1 publication for this product
Publishing research using ab6730? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"