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Product Name 

Rat IgG secondary antibody - H&L

Product type 

Secondary antibodies

Description 

Rabbit polyclonal to Rat IgG - H&L (FITC)

Immunogen 

Rat whole molecule

Tested applications 
(see key)

Flow Cyt, IHC-Fr, IHC-P, IM

Application notes 
(see key)

Recommended dilutions
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

Please refer to the relevant conjugation for dilutions specific for this product:

Conjugate

ELISA

Blotting

Flow Cytometry

IHC

Fluorescein

nd

nd

50 - 200

200 - 1,000

Rhodamine, Texas RedTM

nd

nd

50 - 200

200 - 1,000

R-Phycoerythrin

nd

nd

100 - 200

nd

Cyanines

nd

nd

400

100 - 1,000

Biotin

5,000 - 20,000

3,000 - 10,000

nd

100 - 1,000

Peroxidase

4,000 - 10,000

2,000 - 5,000

nd

200 - 2,000

Alkaline Phosphatase

1,000 - 2,000

500 - 1,000

nd

100 - 1,000

Research areas 

Secondary antibodies >> anti-Rat >> IgG >> Fluorophore >> FITC
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

 

Rat IgG secondary antibody - H&L (ab6730)の別名

Raised in 

Rabbit

Clonality 

Polyclonal

Purity 

IgG fraction

Conjugation notes 

Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons)
Absorption Wavelength: 495 nm
Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

Storage buffer 

0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

2.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

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References for Rat IgG secondary antibody - H&L (ab6730)

Specific publication references   This product has been used in:

Bogomolnaya LM et al. A new enrichment approach identifies genes that alter cell cycle progression in Saccharomyces cerevisiae. Curr Genet 45:350-9 (2004). PubMed: 15022016
If you publish research using ab6730 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to IgG

 

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