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Product Name
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Rat IgG secondary antibody - H&L
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Product type
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Secondary antibodies
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Description
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Rabbit polyclonal to Rat IgG - H&L (FITC)
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Immunogen
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Rat whole molecule
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Tested applications
(see key)
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Flow Cyt, IHC-Fr, IHC-P, IM
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Application notes
(see key)
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Recommended dilutions
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Research areas
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Secondary antibodies >> anti-Rat >> IgG >> Fluorophore >> FITC
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Relevance
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Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
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Rat IgG secondary antibody - H&L (ab6730)の別名 |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons)
Absorption Wavelength: 495 nm
Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
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Storage buffer
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0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Form
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Liquid
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Conjugation
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Fluorescein
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 Rat IgG secondary antibody - H&L について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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References for Rat IgG secondary antibody - H&L (ab6730)
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This product has been used in:
Bogomolnaya LM et al. A new enrichment approach identifies genes that alter cell cycle progression in Saccharomyces cerevisiae. Curr Genet 45:350-9 (2004). PubMed: 15022016
If you publish research using ab6730 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IgG
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