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Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - Fc (AP), pre-adsorbed (ab6258)

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    Overview

    Product name

    Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - Fc (AP), pre-adsorbed
    IgG 抗体 (1691件) 一覧

    Target species

    Rat

    Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG, Rat IgG Fc and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat F(ab’)2 or Bovine, Horse, and Human Serum Proteins.

    Tested applications

    ICC/IF, Dot Blot, WB, ELISA, IHCmore details

    Minimal
    cross-reactivity


    Cow, Horse, Humanmore details

    Immunogen

    Rat IgG F(c) fragment

    Properties

    Form

    Liquid

    Storage instructions

    Store at +4°C. Do not freeze.

    Storage buffer

    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 0.05M Tris HCl, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8

    Concentration

    Concentration information loading...

    Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

    Conjugation notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

    Clonality

    Polyclonal

    Isotype

    IgG

    Research areas

    Secondary antibodies >> anti-Rat >> IgG >> Enzyme >> Alkaline Phos
    Immunology >> Immunoglobulins >> Heavy Chain >> IgG

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab6258 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • ICC/IF

       

    • Dot Blot

       

    • WB

       

    • ELISA

       

    • IHC

       

    Application notes

    Dot: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/5000.
    IHC: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1000 to 1/5000 of the reconstitution concentration is suggested for this product.

    Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    References for Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - Fc (AP), pre-adsorbed (ab6258)

    ab6258 has not yet been referenced specifically in any publications.

    Publishing research using ab6258? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"