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Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - Fc, pre-adsorbed (ab6252)

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Overview

Product name

Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - Fc, pre-adsorbed
IgG 抗体 (1693件) 一覧

Target species

Rat

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins.

Tested applications

WB, ICC/IF, ELISAmore details

Minimal
cross-reactivity


Cow, Horse, Humanmore details

Immunogen

Rat IgG F(c) fragment

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

Concentration

Concentration information loading...

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Clonality

Polyclonal

Isotype

IgG

Research areas

Secondary antibodies >> anti-Rat >> IgG >> Unconjugated
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     WB: 1/2000 - 1/5000.

    WB: 1/2000 - 1/5000.

  • ICC/IF

     ICC/IF: 1/1000 - 1/3000.

    ICC/IF: 1/1000 - 1/3000.

  • ShowHide

    ELISA

     ELISA: 1/10000 - 1/50000.Read more →

    ELISA: 1/10000 - 1/50000.

References for Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - Fc, pre-adsorbed (ab6252)

This product has been referenced in:

  • Gomez AMet al. Proteasome inhibition with bortezomib depletes plasma cells and autoantibodies in experimental autoimmune myasthenia gravis. J Immunol 186:2503-13 (2011). Sandwich ELISA.Read more (PubMed: 21239719) »

See 1 publication for this product

Publishing research using ab6252? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"