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Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - (Fab)'2 (AP), pre-adsorbed (ab6518)

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    Overview

    Product name

    Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - (Fab)'2 (AP), pre-adsorbed
    IgG 抗体 (1693件) 一覧

    Target species

    Rat

    Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG F(ab)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat Fc or Bovine, Horse, and Human Serum Proteins

    Tested applications

    ELISA, IHC, ICC/IF, Dot Blot, WBmore details

    Minimal
    cross-reactivity


    Cow, Horse, Humanmore details

    Immunogen

    Rat IgG F(ab)2 fragment

    Properties

    Form

    Liquid

    Storage instructions

    Store at +4°C. Do not freeze.

    Storage buffer

    Preservative: 0.05% Sodium Azide
    Constituents: 15mg/ml BSA (IgG and protease free), 0.25M Sodium Chloride, 0.01M Tris HCl. pH 8.0

    Concentration

    Concentration information loading...

    Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

    Conjugation notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

    Clonality

    Polyclonal

    Isotype

    IgG

    Research areas

    Secondary antibodies >> anti-Rat >> IgG >> Enzyme >> Alkaline Phos
    Immunology >> Immunoglobulins >> Heavy Chain >> IgG

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab6518 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • ELISA

       ELISA: 1/5000 - 1/50000.

      ELISA: 1/5000 - 1/50000.

    • ShowHide

      IHC

       IHC: Use at an assay dep...Read more →

      IHC: Use at an assay dependent dilution.

    • ICC/IF

       ICC/IF: 1/200 - 1/1000.

      ICC/IF: 1/200 - 1/1000.

    • ShowHide

      Dot Blot

       Dot: Use at an assay dep...Read more →

      Dot: Use at an assay dependent dilution.

    • WB

       WB: 1/5000 - 1/50000.

      WB: 1/5000 - 1/50000.

    References for Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - (Fab)'2 (AP), pre-adsorbed (ab6518)

    ab6518 has not yet been referenced specifically in any publications.

    Publishing research using ab6518? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"