Rat IL-6 ELISA Kit (ab100772)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 40.96 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat IL-6 ELISA Kit
IL-6 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 30 pg/ml -
検出範囲
40.96 pg/ml - 10000 pg/ml -
添加回収試験
119 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 130.2 118% - 138% Plasma 122.8 109% - 138% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat -
製品の概要
Abcam’s IL-6 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat IL-6 in plasma or cell culture supernatants.
This assay employs an antibody specific for Rat IL-6 coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Due to the relatively low abundance of IL-6 in normal rat plasma, this kit may not be suitable for use with this sample type. We have not been able to detect endogenous Rat IL-6 in normal serum with ab100772, only in serum spiked with Rat IL-6.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Rat IL-6 2 vials IL-6 Microplate (12 x 8 wells) 1 unit Recombinant Rat IL-6 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
関連疾患
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
配列類似性
Belongs to the IL-6 superfamily. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
参照データベース
- Entrez Gene: 24498 Rat
- SwissProt: P20607 Rat
- Unigene: 9873 Rat
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (94)
ab100772 は 94 報の論文で使用されています。
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- Potter HG et al. Maternal behaviours and adult offspring behavioural deficits are predicted by maternal TNFα concentration in a rat model of neurodevelopmental disorders. Brain Behav Immun 108:162-175 (2023). PubMed: 36503051
- El Gazzar WB et al. Pioglitazone modulates immune activation and ameliorates inflammation induced by injured renal tubular epithelial cells via PPARγ/miRNA‑124/STAT3 signaling. Biomed Rep 18:2 (2023). PubMed: 36544854
- Guo Y et al. Treadmill Exercise Prevents Cognitive Impairments in Adolescent Intermittent Ethanol Rats by Reducing the Excessive Activation of Microglia Cell in the Hippocampus. Int J Mol Sci 23:N/A (2022). PubMed: 36499029
- Kwon JH et al. Intravenous iron injection does not impair liver function in a rat model of cirrhosis. Ann Palliat Med 11:1687-1699 (2022). PubMed: 35016521