Rap1 Activation Assay Kit (ab212011)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
製品の概要
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製品名
Rap1 Activation Assay Kit -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
種交差性
交差種: Mouse, Rat, Human -
製品の概要
Rap1 Activation Assay Kit (ab212011) uses RalGDS RBD Agarose beads to selectively isolate and pull-down the active form of Rap from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rap is detected by western blot analysis using an anti-Rap1 polyclonal antibody, which reacts with the human, mouse and rat Rap1A and Rap1B proteins.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
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特記事項
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Rap1 (Ras-related protein 1), a 21 kDa protein of the Ras superfamily, exhibits 60% identity to Rap2. Rap2 regulates a variety of biological response pathways that include cell migration, cell adhesion, and embryonic blood vessel formation. Like other small GTPases, Rap1 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rap1 bind specifically to the Rap-binding domain (RBD) of RalGDS to control downstream signaling cascades.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 20 tests 100X GDP 1 x 50µl 100X GTPyS 1 x 50µl 5X Assay/Lysis Buffer 1 x 30ml ab211179 - RalGDS RBD Agarose Beads 1 x 800µl Anti-Rap1 Goat polyclonal antibody 1 x 40µl GTPase immunoblot Positive Control 1 x 100µl -
研究分野
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関連性
RAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known. -
細胞内局在
Cell Membrane; Attached to the membrane by a lipid anchor -
別名
- C21KG
- G 22K
- GTP binding protein smg p21A
see all -
参照データベース
- Entrez Gene: 5906 Human
- Entrez Gene: 5908 Human
- Entrez Gene: 109905 Mouse
- Entrez Gene: 215449 Mouse
- Entrez Gene: 171337 Rat
- Entrez Gene: 295347 Rat
- Omim: 179520 Human
- Omim: RAP1B Human
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関連製品
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab212011 は 2 報の論文で使用されています。
- Kusnadi EP et al. Reprogrammed mRNA translation drives resistance to therapeutic targeting of ribosome biogenesis. EMBO J 39:e105111 (2020). PubMed: 32945574
- Lee H et al. Prominin-1-Radixin axis controls hepatic gluconeogenesis by regulating PKA activity. EMBO Rep 21:e49416 (2020). PubMed: 33030802