Application
ChIP on chip
Sample
Human Cell lysate - nuclear (prostate cancer cells)
Specification
prostate cancer cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Microarray
Positive control
RAD9 has been comfirmed to bind p21 promoter region, so after I precipatated DNA with RAD9 abs, I used precipiated DNA as template, with specific primers for RT-PCR and check p21 promoter region. AB70810 gave positive results as previous reported.
Yes, Following qPCR, I did CHIP microarray with annealing CHIP'd DNA. I got excited data, and now I am doing the following research.
other company RAD9 antibodies, INPUT
Yes, Following qPCR, I did CHIP microarray with annealing CHIP'd DNA. I got excited data, and now I am doing the following research.
other company RAD9 antibodies, INPUT
Negative control
mouse IgG
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Dilution Buffer from INVITROGEN kit
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Legands for Image:
1: 100 bp DNA ladder (BioLabs N3231s)
2: Ab70810
3: RAD9 antibody from BD
4: RAD9 antibody from Novus
5: mIgG
6: INPUT
Primers: specific for p21 promoter region.
2% agarose gel 100v 2.5 hours
1: 100 bp DNA ladder (BioLabs N3231s)
2: Ab70810
3: RAD9 antibody from BD
4: RAD9 antibody from Novus
5: mIgG
6: INPUT
Primers: specific for p21 promoter region.
2% agarose gel 100v 2.5 hours
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 31 2012