Application
ChIP
Sample
Mouse Cell lysate - nuclear (fibroblast)
Specification
fibroblast
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Semiquantitative PCR
Positive control
ccnA2, pcna, chop, grp78 promoter
Negative control
alpha globin promoter
Other product details
Dilution
1/100
Incubation time
0 minute(s)
Additional data
Abcam response
Thank you for your Abreview. I am sorry to hear that this antibody did not chromatin immunoprecipitate in your hands. This antibody is yet to be tested in murine cells or by ChIP. It is possible that this antibody does in fact not immunopreciptiate cross linked chromatin owing to various constraints associated with the binding of the antibody to its epitope under cross linked conditions. Thank you once again for your feedback.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Donati Giacomo
Verified customer
投稿 May 01 2007